Authors was serially monitored lymphocyte subpopulations using monoclonal antibodies and flow cytometry in short-term renal transplant patients treated with cyclosporine(CsA) and low-dose prednisone for the immunologic monitoring. We used
monoclonal
antibodies for the percentage of total T-cell)CD3+, anti-Leu 4), helper/inducer T-cell(CD4+, anti-Leu 3a), suppressor/cytotoxic T-cell(CD8+, anti-Leu 2a) and B-cell(CD19+, anti-Leu 12) in 11 renal transplant patients with the control group of 10
normal
persons, and checked each subject and calculated CD4+/CD8+ cell and CD4+/CD8+ ratio of anticipate rejection.
1) In comparision with stable transplants and normal control, there were no significant statistical differences in CD3+, CD4+, CD8+ cell and CD4+/CD8+ ratio preoperatively(p>0.05).
2) There was no significant statistical difference in CD3+ cell in table recipients postoperatively(P>0.05)
3) In stable recipients, the levels of CD4+ was decreased, CD8+ increased and CD4+/CD8+ ratio decreased significantly at post-transplant 30th day(p<0.05).
4) In a case of acute rejection, CD4+/CD8+ ratio was increased due to increase of CD4+ preoperatively and at time of acute rejection.
5) There was no significant statistical difference of B-cell pre-and post-operatively in stable recipients and a case of acute rejection(p<0.05).
Those suggested that decreased ratio of CD4+/CD8+ cell within post-transplant 3 months was due to the increase of CD8+ cell relatively in stable recipients. Suppressor T-cell may contribute to the immune hyporesponsiveness and the maintenance of
immnune
tolerance in short-term renal transplant recipients.
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